EBTT 2014

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought — each participant submitting an abstract was also required to give a short oral presentation of the work. This course was accredited also by the Medical Chamber of Slovenia, so active and passive participants received 20 CME credits.

Read all about EBTT in an article published by MediaResearch Ltd (2012). International Innovation is the leading global dissemination resource for the wider scientific, technology and research communities — more at www.researchmedia.eu.

Organised by

University of Ljubljana · Institute of Oncology, Ljubljana · COST Action TD 1104

Organising committee

Chair: Peter Kramar
Members: Maša Kandušer, Matej Kranjc, Marija Marčan, Tadeja Forjanič, Duša Hodžić, Lea Vukanović

Course content

Key lectures & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties of tissues; temperature & conductivity changes.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for electroporation of cells in vitro and in vivo.

Devices & electrodes

Devices & electrodes for in vitro / in vivo work; tissue models & their experimental validation.

Electrochemotherapy of tumours

Basic mechanisms, preclinical studies, clinical use, current developments & future perspectives.

Gene transfection

Gene transfection in vitro / in vivo; clinical use in human medicine.

Transdermal drug delivery

Electroporation for gene delivery in the skin; DNA vaccines.

Additional topics

Colorectal liver metastases ECT; electrochemical processes in HV PEF; systemic ECT; food & biomass processing; MREIT field monitoring.

Additional invited talks

Ibrahim EdhemovićThe role of electrochemotherapy in treatment of colorectal liver metastases — has the time come?
Gintautas SaulisElectrochemical processes occurring during exposure of cells by high-voltage pulsed electric fields and their consequences
Declan SodenExpanding electrochemotherapy from local to systemic tumour control
Eugène VorobievElectroporation based technologies for food and biomass processing
Matej KranjcMonitoring of electric field distribution during electroporation pulse delivery by means of MREIT: from concept to first in vivo results

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič

Co-DirectorLluis M. Mir

Faculty members & lecturers

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
Eberhard NeumannUniversity of Bielefeld, Germany
Véronique PréatCatholic University of Louvain, Brussels, Belgium
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Justin TeissiéCNRS & IPBS, Toulouse, France
P. Thomas VernierUniversity of Southern California, USA

Invited lecturers

Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
Ibrahim EdhemovićUniversity of Ljubljana, Slovenia
Tadej KotnikUniversity of Ljubljana, Slovenia
Matej KranjcUniversity of Ljubljana, Slovenia
Gintautas SaulisSemiconductor Physics Institute, Lithuania
Marie-Pierre RolsCNRS & IPBS, Toulouse, France
Declan SodenCork Cancer Research Centre, Cork, Ireland
Mounir TarekUniversité Henri-Poincaré, Nancy, France
Eugène VorobievUniversité de Technologie de Compiègne, France

Hands-on

Practical work & exercises

Wet-lab, computer-modelling and e-learning sessions. Participants selected up to three sessions from wet lab and modelling, plus computer simulation and e-learning. Exercise sheets (PDF) as distributed to participants.

S1	Laboratory safety	PDF ↓
L1	The influence of Mg²⁺ ions on gene electrotransfer efficiency	PDF ↓
L2	Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM	PDF ↓
L3	Electropermeabilization detection with propidium iodide in plated cells	PDF ↓
L4	Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS	PDF ↓
L5	Irreversible electroporation of bacterial cells	PDF ↓
L6	Electroporation of planar lipid bilayers	PDF ↓
L7	E. coli inactivation in a continuous flow system	PDF ↓
L8	Analysis of electric field orientations on gene electrotransfer — efficiency and visualization at the membrane level	PDF ↓
L9	Skin electroporation	PDF ↓
C1	Electrochemotherapy treatment planning: optimization of voltage & electrode position	PDF ↓
C2	Numerical modelling of thermal effects during irreversible electroporation treatments	PDF ↓
C3	Molecular dynamics simulations of membrane electroporation	PDF ↓
E1	Electroporation of cells and tissues — interactive e-learning	PDF ↓

Abstracts & publication

Abstract instructions & proceedings

PDFInstructions for Authors PDFAbstract Submission Form DOCAbstract Template PDFJMB Author Guidelines

Workshop fee (630 EUR) included attendance to lectures, book of abstracts and course material, lunches and social events, and certificate of attendance and examination (5 ECTS credits and 20 CME if required). The postgraduate course was co-financed by the European Social Fund and the Ministry for Education, Science and Sport, in the framework of the Operational Programme for Human Resources Development 2007–2013.

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros
nina@albatros-bled.com
tel +386 4 5780 350 · fax +386 4 5780 355